M. Kurimoto, L. D. Bastin, D. Fredrickson, P. N. Gustafson, S.-H Jang, W. Kaminsky, S. Lovell, C. A. Mitchell, J. Chmielewski, B. Kahr. Intrasectoral zoning of proteins and nucleotides in simple crystalline hosts.
Oriented gases of biopolymers in simple, single crystal hosts might be used
to measure anisotropic molecular properties of analytes that could not otherwise
be crystallized. Here we show two types of crystals as examples of the single
crystal matrix isolation of biopolymers: green fluorescent protein in
alpha-lactose monohydrate as a model system for studying the kinetic
stabilization of biopharmaceuticals, and adenosine phosphates in potassium
dihydrogen phosphate, a first step in the matrix isolation of oligonucleotides.In
each case, the hosts undergo compositional zoning - both intersectoral -
during growth from solution. Intrasectoral zoning is evident by the selective
luminescence of adjacent vicinal slopes of growth active hillocks. Nucleotides
furthermore distinguish between symmetry related growth sectors
enantioselectively.